Microfluidic chips can simulate the three-dimensional growth environment of cells in the body. The interaction between cells can be studied by controlling the external conditions such as material concentration, solution temperature and pH value.

     Cell-cell interactions are direct interactions between cells and play an important role in the development and function of multicellular organisms, and they maintain their homeostasis under aging and pathological conditions. Cell-cell interactions are involved in many physiological and pathological processes, such as embryonic development, wound healing, tumor invasion and metastasis.

     The microfluidic chip has the characteristics of flexible design and can cultivate many kinds of cells simultaneously in a large number of microchannels. Through the introduction of 3D extracellular matrix, the generation and concentration of biochemical factors and signaling molecules can be monitored and controlled in real time, Study on the Interactions. Current microfluidic chip research cell-cell interaction is the most commonly used method of cell co-culture.